International researchers have sent a set of human muscle cells into space to see how their growth is impacted in microgravity. The muscle chips – bioengineered packages of cells mimicking the structure of human muscles – were sent to the International Space Station to grow for a week. When they came back, the researchers compared them to similar muscle chips left on Earth, and they say the cells grown in space showed various signs of dysfunction, including gene activities resembling muscles with sarcopenia. Sarcopenia usually only affects people aged 60 and over, the researchers say, however, it appears this process may be rapidly accelerated in space. Muscle chips in space that were spiked with sarcopenia drugs were able to better resemble the chips that never left Earth, the researchers say.
Funder: This work was supported in part by grants to N.F.H. from the US
National Institutes of Health (R01 HL142718, R41 HL170875, and
R21 HL172096-01), the US Department of Veterans Affairs
(1I01BX004259, 5I01RX001222, and 1I21RX004898), the National
Science Foundation (1829534 and 2227614), the Center for
the Advancement of Science in Space (CASIS, 80JSC018M0005),
and the American Heart Association (20IPA35360085 and
20IPA35310731) and by grants to T.A.R. fromthe US National Institutes
of Health (R01 AG068667 and P01 AG036695) and the US
Department of Veterans Affairs (I01BX002324 and I01RX001222).
N.F.H. is a recipient of a Research Career Scientist award (IK6
BX006309) from the Department of Veterans Affairs
Media release
From: Cell Press
Peer-reviewed Experimental study CellsSpace-trekking muscle tests drugs for microgravity-induced muscle impairmentA gentle rumble ran under Ngan Huang’s feet as a rocket carrying her research—live, human muscle cells grown on scaffolds fixed on tiny chips—lifted off, climbed, and disappeared into the sky to the International Space Station National Laboratory. These chips would help Huang better understand muscle impairment, often seen in astronauts and older adults, and test drugs to counter the condition.Now, the results are back. Reporting in a study published July 25 in the journal Stem Cell Reports, Huang’s team showed that space-travelling muscle had metabolic changes that indicate impaired muscle regeneration and gene activities associated to age-related muscle loss called sarcopenia. But drug treatment partially prevented microgravity’s adverse effects.“Space is a really unique environment that accelerates qualities associated with aging and also impairs many healthy processes,” says Huang (@NganHuang), an associate professor at Stanford University. “Astronauts come back with muscle atrophy, or a reduction of muscle function, because the muscle isn’t being actively used in the absence of gravity. As space travel becomes more common and available to civilians, it’s important to understand what happens to our muscle in microgravity.”To understand the effects of microgravity on muscles, the researchers launched muscle chips—bioengineered packages of oriented muscle cells on patterned biomaterials that mimic the structure of real muscles—into space to grow for seven days under astronauts’ care.When the researchers compared muscle cells grown in microgravity to those grown on Earth, they found impaired muscle fiber formation. They also discovered differences in their gene activity and protein profile. Genes related to mitochondrial function, which muscles get their energy from, were compromised, and genes associated with fat formation were boosted. These findings suggest microgravity can lead to dysfunctions in muscle regeneration.Space-traveled muscles also have gene activities that somewhat resemble muscles with sarcopenia, which most commonly affects people ages 60 and older.“We think our research on muscle chips in microgravity may have broader implications on sarcopenia,” says Huang. “Sarcopenia usually takes decades to develop on Earth, and we think that microgravity may have some ability to accelerate the disease process in orders of days.”In a proof-of-concept experiment to test the muscle chip for drug screening, the astronauts spiked the chips with drugs to treat sarcopenia or enhance muscle regeneration. The treatment partially mitigated some of the negative effects of microgravity on the muscles, preventing a metabolic shift to fat formation. Looking into gene activity patterns, the drug-treated muscle in microgravity is more similar to samples from Earth than untreated samples in microgravity.Because space research is labor and resource intensive, the current study is a one-time experiment, and a limited number of samples were allowed to board the rocket. The scientists are now deploying equipment that simulates microgravity to overcome some of those limitations and aid their research in space. Huang’s muscle chips are scheduled to embark on another space journey in 2025 to continue the research on identifying drugs for treating microgravity-induced impairment in muscle regeneration.“This concept of engineered tissue chip platform in microgravity is a potentially transformative tool that could allow us to study a variety of diseases and do drug screening without animal or human subjects,” says Huang.