Liu, Y., Heying, E. & Tanumihardjo, S. A. History, global distribution, and nutritional importance of citrus fruits. Compr. Rev. Food Sci. Food Saf. 11, 530–545 (2012).ArticleÂ
Google ScholarÂ
Alvarez, S., Rohrig, E., SolÃs, D. & Thomas, M. H. Citrus greening disease (Huanglongbing) in Florida: Economic impact, management and the potential for biological control. Agric. Res. 5, 109–118 (2016).ArticleÂ
Google ScholarÂ
Bové, J. M. Huanglongbing: A destructive, newly-emerging, century-old disease of citrus. J. Plant Pathol. 88, 7–37 (2006).
Google ScholarÂ
Lee, J. A. et al. Asymptomatic spread of Huanglongbing and implications for disease control. Proc. Natl. Acad. Sci. U. S. A. 112, 7605–7610 (2015).ArticleÂ
ADSÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Manjunath, K. L., Halbert, S. E., Ramadugu, C., Webb, S. & Lee, R. F. Detection of ‘Candidatus Liberibacter asiaticus’ in Diaphorina citri and its importance in the management of citrus Huanglongbing in Florida. Phytopathology® 98, 387–396 (2008).ArticleÂ
PubMedÂ
Google ScholarÂ
Gottwald, T. R. Current epidemiological understanding of citrus Huanglongbing. Annu. Rev. Phytopathol. 48, 119–139 (2010).ArticleÂ
PubMedÂ
Google ScholarÂ
Boina, D. R. & Bloomquist, J. R. Chemical control of the Asian citrus psyllid and of Huanglongbing disease in citrus. Pest Manag. Sci. 71, 808–823 (2015).ArticleÂ
PubMedÂ
Google ScholarÂ
Ghosh, D. et al. Huanglongbing pandemic: Current challenges and emerging management strategies. Plants 12, 160 (2022).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Ginnan, N. A. et al. Disease-induced microbial shifts in citrus indicate microbiome-derived responses to Huanglongbing across the disease severity spectrum. Phytobiomes J. 4, 375–387 (2020).ArticleÂ
Google ScholarÂ
Zhong, Y. et al. Comparative transcriptome and iTRAQ proteome analyses of citrus root responses to Candidatus Liberibacter asiaticus infection. PLoS One 10, e0126973 (2015).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Li, B., Zhang, Y., Qiu, D., Francis, F. & Wang, S. Comparative proteomic analysis of sweet orange petiole provides insights into the development of Huanglongbing symptoms. Front. Plant Sci. 12, 656997 (2021).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Yang, C. et al. Metagenomic analysis reveals the mechanism for the observed increase in antibacterial activity of penicillin against uncultured bacteria Liberibacter asiaticus relative to oxytetracycline in planta. Antibiotics (Basel) 9, 874 (2020).ArticleÂ
PubMedÂ
Google ScholarÂ
Zhang, M. et al. Effective antibiotics against ‘Candidatus Liberibacter asiaticus’ in HLB-affected citrus plants identified via the graft-based evaluation. PLoS One 9, e111032 (2014).ArticleÂ
ADSÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Hu, J. & Wang, N. Evaluation of the spatiotemporal dynamics of oxytetracycline and its control effect against citrus Huanglongbing via trunk injection. Phytopathology 106, 1495–1503 (2016).ArticleÂ
PubMedÂ
Google ScholarÂ
Zuñiga, C. et al. Linking metabolic phenotypes to pathogenic traits among ‘Candidatus Liberibacter asiaticus’ and its hosts. NPJ Syst. Biol. Appl. 6, 24 (2020).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Schauer, N. & Fernie, A. R. Plant metabolomics: Towards biological function and mechanism. Trends Plant Sci. 11, 508–516 (2006).ArticleÂ
PubMedÂ
Google ScholarÂ
Macel, M., Van Dam, N. M. & Keurentjes, J. J. B. Metabolomics: The chemistry between ecology and genetics. Mol. Ecol. Resour. 10, 583–593 (2010).ArticleÂ
PubMedÂ
Google ScholarÂ
Yao, L. et al. Proteomic and metabolomic analyses provide insight into the off-flavour of fruits from citrus trees infected with ‘Candidatus Liberibacter asiaticus’. Hortic. Res. 6, 31 (2019).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Hijaz, F. M. et al. An HPLC-MS characterization of the changes in sweet orange leaf metabolite profile following infection by the bacterial pathogen Candidatus Liberibacter asiaticus. PLoS One 8, e79485 (2013).ArticleÂ
ADSÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Aksenov, A. A. et al. Detection of Huanglongbing disease using differential mobility spectrometry. Anal. Chem. 86, 2481–2488 (2014).ArticleÂ
PubMedÂ
Google ScholarÂ
Protsyuk, I. et al. 3D molecular cartography using LC-MS facilitated by Optimus and ’ili software. Nat. Protoc. 13, 134–154 (2018).ArticleÂ
PubMedÂ
Google ScholarÂ
Kapono, C. A. et al. Creating a 3D microbial and chemical snapshot of a human habitat. Sci. Rep. 8, 3669 (2018).ArticleÂ
ADSÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Floros, D. J. et al. Mass spectrometry based molecular 3D-cartography of plant metabolites. Front. Plant Sci. 8, 429 (2017).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Bouslimani, A. et al. Molecular cartography of the human skin surface in 3D. Proc. Natl. Acad. Sci. U. S. A. 112, E2120–E2129 (2015).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Wang, M. et al. Sharing and community curation of mass spectrometry data with global natural products social molecular networking. Nat. Biotechnol. 34, 828–837 (2016).ArticleÂ
ADSÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Aron, A. T. et al. Reproducible molecular networking of untargeted mass spectrometry data using GNPS. Nat. Protoc. https://doi.org/10.1038/s41596-020-0317-5 (2020).ArticleÂ
PubMedÂ
Google ScholarÂ
Watrous, J. et al. Mass spectral molecular networking of living microbial colonies. Proc. Natl. Acad. Sci. U. S. A. 109, E1743–E1752 (2012).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
KEGG PATHWAY: Phenylpropanoid biosynthesis – Citrus sinensis (Valencia orange). https://www.genome.jp/kegg-bin/show_pathway?cit00940.Feng, G., Ai, X., Yi, H., Guo, W. & Wu, J. Genomic and transcriptomic analyses of Citrus sinensis varieties provide insights into Valencia orange fruit mastication trait formation. Hortic. Res. 8, 218 (2021).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Chen, Q. et al. Metabolomic analysis revealed distinct physiological responses of leaves and roots to Huanglongbing in a citrus rootstock. Int. J. Mol. Sci. 23, 9242 (2022).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Chin, E. L., Mishchuk, D. O., Breksa, A. P. & Slupsky, C. M. Metabolite signature of Candidatus Liberibacter asiaticus infection in two citrus varieties. J. Agric. Food Chem. 62, 6585–6591 (2014).ArticleÂ
PubMedÂ
Google ScholarÂ
Deng, H. et al. Comparative leaf volatile profiles of two contrasting mandarin cultivars against Liberibacter asiaticus infection illustrate Huanglongbing tolerance mechanisms. J. Agric. Food Chem. 69, 10869–10884 (2021).ArticleÂ
PubMedÂ
Google ScholarÂ
Padhi, E. M. T. et al. Metabolome and microbiome signatures in the roots of citrus affected by Huanglongbing. Phytopathology 109, 2022–2032 (2019).ArticleÂ
PubMedÂ
Google ScholarÂ
Blacutt, A. et al. An in vitro pipeline for screening and selection of citrus-associated microbiota with potential anti-‘Candidatus Liberibacter asiaticus’ properties. Appl. Environ. Microbiol. https://doi.org/10.1128/AEM.02883-19 (2020).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Irigoyen, S. et al. Plant hairy roots enable high throughput identification of antimicrobials against Candidatus Liberibacter spp. Nat. Commun. 11, 5802 (2020).ArticleÂ
ADSÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Li, J. et al. The in planta effective concentration of oxytetracycline against ’ Liberibacter asiaticus’ for suppression of citrus Huanglongbing. Phytopathology 109, 2046–2054 (2019).ArticleÂ
PubMedÂ
Google ScholarÂ
Hu, J., Jiang, J. & Wang, N. Control of CITRUS Huanglongbing via trunk injection of plant defense activators and antibiotics. Phytopathology 108, 186–195 (2018).ArticleÂ
PubMedÂ
Google ScholarÂ
Munir, S. et al. Unraveling the metabolite signature of citrus showing defense response towards Candidatus Liberibacter asiaticus after application of endophyte Bacillus subtilis L1–21. Microbiol. Res. 234, 126425 (2020).ArticleÂ
PubMedÂ
Google ScholarÂ
Hung, W.-L. & Wang, Y. A targeted mass spectrometry-based metabolomics approach toward the understanding of host responses to Huanglongbing disease. J. Agric. Food Chem. 66, 10651–10661 (2018).ArticleÂ
PubMedÂ
Google ScholarÂ
Suh, J. H., Tang, X., Zhang, Y., Gmitter, F. G. Jr. & Wang, Y. Metabolomic analysis provides new insight into tolerance of Huanglongbing in Citrus. Front. Plant Sci. 12, 710598 (2021).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Nuutila, A. M., Kammiovirta, K. & Oksman-Caldentey, K.-M. Comparison of methods for the hydrolysis of flavonoids and phenolic acids from onion and spinach for HPLC analysis. Food Chem. 76, 519–525. https://doi.org/10.1016/s0308-8146(01)00305-3 (2002).ArticleÂ
Google ScholarÂ
Pourcel, L., Routaboul, J., Cheynier, V., Lepiniec, L. & Debeaujon, I. Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends Plant Sci. 12, 29–36. https://doi.org/10.1016/j.tplants.2006.11.006 (2007).ArticleÂ
PubMedÂ
Google ScholarÂ
Mandadi, K. et al. Hairy roots to the rescue: Speeding up discovery for HLB Management. Citrograph 11, 20–22 (2020).
Google ScholarÂ
Kennedy, J. P. et al. A perspective on current therapeutic molecule screening methods against ’ Liberibacter asiaticus’, the presumed causative agent of citrus Huanglongbing. Phytopathology 113, 1171–1179 (2023).ArticleÂ
PubMedÂ
Google ScholarÂ
Zhang, M. et al. Field evaluation of integrated management for mitigating citrus Huanglongbing in Florida. Front. Plant Sci. 9, 1890 (2018).ArticleÂ
PubMedÂ
Google ScholarÂ
Davis, M. J., Mondal, S. N., Chen, H., Rogers, M. E. & Brlansky, R. H. Co-cultivation of ‘Candidatus Liberibacter asiaticus’ with actinobacteria from citrus with Huanglongbing. Plant Dis. 92, 1547–1550 (2008).ArticleÂ
PubMedÂ
Google ScholarÂ
Leonard, M. T., Fagen, J. R., Davis-Richardson, A. G., Davis, M. J. & Triplett, E. W. Complete genome sequence of Liberibacter crescens BT-1. Stand. Genom. Sci. 7, 271–283 (2012).ArticleÂ
Google ScholarÂ
Barnett, M. J., Solow-Cordero, D. E. & Long, S. R. A high-throughput system to identify inhibitors of Liberibacter asiaticus transcription regulators. Proc. Natl. Acad. Sci. U. S. A. 116, 18009–18014 (2019).ArticleÂ
ADSÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Jain, M. et al. Is a cultured surrogate for functional genomics of uncultured pathogenic ‘Liberibacter’ spp. and is naturally competent for transformation. Phytopathology 109, 1811–1819 (2019).ArticleÂ
PubMedÂ
Google ScholarÂ
dos Santos, W. D. et al. Soybean (Glycine max) root lignification induced by ferulic acid. The possible mode of action. J. Chem. Ecol. 34, 1230–1241 (2008).ArticleÂ
PubMedÂ
Google ScholarÂ
Cushnie, T. P. T. & Lamb, A. J. Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents 26, 343–356 (2005).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Ou, S. & Kwok, K.-C. Ferulic acid: Pharmaceutical functions, preparation and applications in foods. J. Sci. Food Agric. 84, 1261–1269 (2004).ArticleÂ
Google ScholarÂ
Paczkowski, J. E. et al. Flavonoids suppress virulence through allosteric inhibition of quorum-sensing receptors. J. Biol. Chem. 292, 4064–4076 (2017).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Borges, A., Saavedra, M. J. & Simões, M. The activity of ferulic and gallic acids in biofilm prevention and control of pathogenic bacteria. Biofouling 28, 755–767 (2012).ArticleÂ
PubMedÂ
Google ScholarÂ
Li, A.-P., He, Y.-H., Zhang, S.-Y. & Shi, Y.-P. Antibacterial activity and action mechanism of flavonoids against phytopathogenic bacteria. Pestic. Biochem. Physiol. 188, 105221 (2022).ArticleÂ
PubMedÂ
Google ScholarÂ
Borges, A., Ferreira, C., Saavedra, M. J. & Simões, M. Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microb. Drug Resist. 19, 256–265 (2013).ArticleÂ
PubMedÂ
Google ScholarÂ
Archer, L., Kunwar, S., Alferez, F., Batuman, O. & Albrecht, U. Trunk injection of oxytetracycline for Huanglongbing management in mature grapefruit and sweet orange trees. Phytopathology 113, 1010–1021 (2023).ArticleÂ
PubMedÂ
Google ScholarÂ
Blaustein, R. A., Lorca, G. L. & Teplitski, M. Challenges for managing Candidatus Liberibacter spp. (Huanglongbing disease pathogen): Current control measures and future directions. Phytopathology 108, 424–435 (2018).ArticleÂ
PubMedÂ
Google ScholarÂ
Gardner, C. L. et al. Assessment of unconventional antimicrobial compounds for the control of ‘Candidatus Liberibacter asiaticus’, the causative agent of citrus greening disease. Sci. Rep. 10, 5395 (2020).ArticleÂ
ADSÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Archer, L., Qureshi, J. & Albrecht, U. Efficacy of trunk injected imidacloprid and oxytetracycline in managing huanglongbing and Asian citrus psyllid in infected sweet orange (Citrus sinensis) trees. Collect. FAO Agric. 12, 1592 (2022).
Google ScholarÂ
Xia, J. & Wishart, D. S. Using MetaboAnalyst 3.0 for comprehensive metabolomics data analysis. Curr. Protoc. Bioinform. 55, 14.10.1-14.10.91 (2016).ArticleÂ
Google ScholarÂ
Hackstadt, A. J. & Hess, A. M. Filtering for increased power for microarray data analysis. BMC Bioinform. https://doi.org/10.1186/1471-2105-10-11 (2009).ArticleÂ
Google ScholarÂ
Wold, S., Sjöström, M. & Eriksson, L. PLS-regression: A basic tool of chemometrics. Chemom. Intell. Lab. Syst. 58, 109–130 (2001).ArticleÂ
Google ScholarÂ
Breiman, L. Random forests. Mach. Learn. 45, 5–32 (2001).ArticleÂ
Google ScholarÂ
Nothias, L. F. et al. Feature-based molecular networking in the GNPS analysis environment. Nat. Methods https://doi.org/10.1101/812404 (2019).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Kyselka, J. et al. Antifungal polyamides of hydroxycinnamic acids from sunflower bee pollen. J. Agric. Food Chem. 66, 11018–11026. https://doi.org/10.1021/acs.jafc.8b03976 (2018).ArticleÂ
PubMedÂ
Google ScholarÂ
Schellenberger, J. et al. Quantitative prediction of cellular metabolism with constraint-based models: The COBRA Toolbox v2.0. Nat. Protoc. 6, 1290–1307 (2011).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Zheng, Z. et al. Unusual five copies and dual forms of nrdB in ‘Candidatus Liberibacter asiaticus’: Biological implications and PCR detection application. Sci. Rep. 6, 39020 (2016).ArticleÂ
ADSÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Mafra, V. et al. Reference genes for accurate transcript normalization in citrus genotypes under different experimental conditions. PLoS One 7, e31263 (2012).ArticleÂ
ADSÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ