Shuryak, I. et al. A machine learning method for improving the accuracy of radiation biodosimetry by combining data from the dicentric chromosomes and micronucleus assays. Sci. Rep. 12, 21077 (2022).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Blakely, W. F., Prasanna, P. G., Grace, M. B. & Miller, A. C. Radiation exposure assessment using cytological and molecular biomarkers. Radiat. Prot. Dosimetry 97, 17–23 (2001).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Testa, A., Palma, V. & Patrono, C. Dicentric chromosome assay (DCA) and cytokinesis-block micronucleus (CBMN) assay in the field of biological dosimetry. Methods Mol. Biol. 2031, 105–119 (2019).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Dainiak, N., Waselenko, J. K., Armitage, J. O., MacVittie, T. J. & Farese, A. M. The hematologist and radiation casualties. Hematol. Am. Soc. Hematol. Educ. Progr. 2003, 473–496 (2003).ArticleÂ
Google ScholarÂ
Waselenko, J. K. et al. Medical management of the acute radiation syndrome: Recommendations of the Strategic National Stockpile Radiation Working Group. Ann. Intern. Med. 140, 1037–1051 (2004).ArticleÂ
PubMedÂ
Google ScholarÂ
Schuening, F. G. et al. Effect of recombinant human granulocyte colony-stimulating factor on hematopoiesis of normal dogs and on hematopoietic recovery after otherwise lethal total body irradiation. Blood 74, 1308–1313 (1989).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Hofer, M., PospÃÅ¡il, M., Komůrková, D. & Hoferová, Z. Granulocyte colony-stimulating factor in the treatment of acute radiation syndrome: A concise review. Molecules 19, 4770–4778 (2014).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
MacVittie, T. J. et al. The effect of radiation dose and variation in Neupogen® initiation schedule on the mitigation of myelosuppression during the concomitant GI-ARS and H-ARS in a nonhuman primate model of high-dose exposure with marrow sparing. Health Phys. 109, 427–439 (2015).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Garty, G. et al. The decade of the RABiT (2005–15). Radiat. Prot. Dosimetry 172, 201–206 (2016).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Repin, M., Pampou, S., Karan, C., Brenner, D. J. & Garty, G. RABiT-II: Implementation of a high-throughput micronucleus biodosimetry assay on commercial biotech robotic systems. Radiat. Res. 187, 502–508 (2017).ArticleÂ
ADSÂ
Google ScholarÂ
Royba, E. et al. RABiT-II-DCA: A fully-automated dicentric chromosome assay in multiwell plates. Radiat. Res. 192, 311–323 (2019).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Ravi, M., Nivedita, K. & Pai, G. M. Chromatin condensation dynamics and implications of induced premature chromosome condensation. Biochimie 95, 124–133 (2013).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Pantelias, A. & Terzoudi, G. I. Development of an automatable micro-PCC biodosimetry assay for rapid individualized risk assessment in large-scale radiological emergencies. Mutat. Res. Toxicol. Environ. Mutagen. 836, 65–71 (2018).ArticleÂ
CASÂ
Google ScholarÂ
Yadav, U., Bhat, N. N., Shirsaath, K. B., Mungse, U. S. & Sapra, B. K. Refined premature chromosome condensation (G0-PCC) with cryo-preserved mitotic cells for rapid radiation biodosimetry. Sci. Rep. 11, 13498 (2021).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Hernansaiz-Ballesteros, R. D., Földi, C., Cardelli, L., Nagy, L. G. & Csikász-Nagy, A. Evolution of opposing regulatory interactions underlies the emergence of eukaryotic cell cycle checkpoints. Sci. Rep. 11, 11122 (2021).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Prasanna, P. G. S. & Blakely, W. F. Premature chromosome condensation in human resting peripheral blood lymphocytes for chromosome aberration analysis using specific whole-chromosome DNA hybridization probes. Methods Mol. Biol. 291, 49–57 (2005).CASÂ
PubMedÂ
Google ScholarÂ
Prasanna, P. G., Escalada, N. D. & Blakely, W. F. Induction of premature chromosome condensation by a phosphatase inhibitor and a protein kinase in unstimulated human peripheral blood lymphocytes: A simple and rapid technique to study chromosome aberrations using specific whole-chromosome DNA hybridization probes. Mutat. Res. Toxicol. Environ. Mutagen. 466, 131–141 (2000).ArticleÂ
CASÂ
Google ScholarÂ
Gotoh, E. G2 premature chromosome condensation/chromosome aberration assay: Drug-induced premature chromosome condensation (PCC) protocols and cytogenetic approaches in mitotic chromosome and interphase chromatin for radiation biology. Methods Mol. Biol. 1984, 47–60 (2019).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Gelens, L., Qian, J., Bollen, M. & Saurin, A. T. The importance of kinase-phosphatase integration: lessons from mitosis. Trends Cell Biol. 28, 6–21 (2018).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Kishimoto, T. Entry into mitosis: A solution to the decades-long enigma of MPF. Chromosoma 124, 417–428 (2015).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Hara, M. et al. Greatwall kinase and Cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor. Nat. Commun. 3, 1059 (2012).ArticleÂ
ADSÂ
PubMedÂ
Google ScholarÂ
Shintomi, K., Takahashi, T. S. & Hirano, T. Reconstitution of mitotic chromatids with a minimum set of purified factors. Nat. Cell Biol. 17, 1014–1023 (2015).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Shintomi, K. Making mitotic chromosomes in a test tube. Epigenomes 6, 1–13 (2022).ArticleÂ
Google ScholarÂ
Terakawa, T. et al. The condensin complex is a mechanochemical motor that translocates along DNA. Science 358, 672–676 (2017).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Hirano, T. Condensins: Universal organizers of chromosomes with diverse functions. Genes Dev. 26, 1659–1678 (2012).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Bazile, F., St-Pierre, J. & D’Amours, D. Three-step model for condensin activation during mitotic chromosome condensation. Cell Cycle 9, 3263–3275 (2010).ArticleÂ
Google ScholarÂ
Kschonsak, M. & Haering, C. H. Shaping mitotic chromosomes: From classical concepts to molecular mechanisms. BioEssays 37, 755–766 (2015).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Kagami, Y., Ono, M. & Yoshida, K. Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis. Sci. Rep. 7, 5583 (2017).ArticleÂ
ADSÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Tsuji, S. & Kanda, R. Chemically induced premature chromosome condensation in short-term cultured human peripheral lymphocytes: applications to biodosimetry. Biotech. Histochem. 82, 29–34 (2007).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Sommer, S. et al. The rapid interphase chromosome assay (RICA) implementation: Comparison with other PCC methods. Nukleonika 60, 933–941 (2015).ArticleÂ
Google ScholarÂ
Walev, I. et al. Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O. Proc. Natl. Acad. Sci. 98, 3185–3190 (2001).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Babiychuk, E. B., Monastyrskaya, K., Potez, S. & Draeger, A. Blebbing confers resistance against cell lysis. Cell Death Differ. 18, 80–89 (2011).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Teng, K. W. et al. Labeling proteins inside living cells using external fluorophores for microscopy. eLife 5, e20378 (2016).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Vigneron, S. et al. Cyclin A-cdk1-dependent phosphorylation of bora is the triggering factor promoting mitotic entry. Dev. Cell 45, 637-650.e7 (2018).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
St-Pierre, J. et al. Polo kinase regulates mitotic chromosome condensation by hyperactivation of condensin DNA supercoiling activity. Mol. Cell 34, 416–426 (2009).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Abe, S. et al. The initial phase of chromosome condensation requires Cdk1-mediated phosphorylation of the CAP-D3 subunit of condensin II. Genes Dev. 25, 863–874 (2011).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Gong, D. & Ferrell, J. E. The roles of Cyclin A2, B1, and B2 in early and late mitotic events. Mol. Biol. Cell 21, 3149–3161 (2010).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Xin, G. et al. Aurora B regulates PP1γ-Repo-Man interactions to maintain the chromosome condensation state. J. Biol. Chem. 295, 14780–14788 (2020).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Liu, Q. & Ruderman, J. V. Aurora A, mitotic entry, and spindle bipolarity. Proc. Natl. Acad. Sci. 103, 5811–5816 (2006).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Wilkins, B. J. et al. A cascade of histone modifications induces chromatin condensation in mitosis. Science 343, 77–80 (2014).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
Google ScholarÂ
Mochida, S. & Hunt, T. Protein phosphatases and their regulation in the control of mitosis. EMBO Rep. 13, 197–203 (2012).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Schmidhuber, J. Deep learning in neural networks: An overview. Neural Netw. 61, 85–117 (2015).ArticleÂ
PubMedÂ
Google ScholarÂ
LeCun, Y., Bengio, Y. & Hinton, G. Deep learning. Nature 521, 436–444 (2015).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
Google ScholarÂ
Krizhevsky, A., Sutskever, I. & Hinton, G. E. ImageNet classification with deep convolutional neural networks. Commun. ACM 60, 84–90 (2017).ArticleÂ
Google ScholarÂ
Vicar, T. et al. DeepFoci: Deep learning-based algorithm for fast automatic analysis of DNA double-strand break ionizing radiation-induced foci. Comput. Struct. Biotechnol. J. 19, 6465–6480 (2021).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Shen, X. et al. High-precision automatic identification method for dicentric chromosome images using two-stage convolutional neural network. Sci. Rep. 13, 2124 (2023).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Jang, S. et al. Feasibility study on automatic interpretation of radiation dose using deep learning technique for dicentric chromosome assay. Radiat. Res. 195(2), 163–172 (2020).ArticleÂ
Google ScholarÂ
Lecun, Y. & Yoshua, B. Convolutional networks for images, speech, and time-series. in The Handbook of Brain Theory and Neural Networks (MIT Press, 1995).LeCun, Y., Kavukcuoglu, K. & Farabet, C. Convolutional networks and applications in vision. in Proceedings of 2010 IEEE International Symposium on Circuits and Systems 253–256 (IEEE, 2010).Satyamitra, M. M. et al. The NIAID/RNCP biodosimetry program: An overview. Cytogenet. Genome Res. 163, 89–102 (2023).ArticleÂ
PubMedÂ
Google ScholarÂ
Simon, S. L., Bouville, A. & Kleinerman, R. Current use and future needs of biodosimetry in studies of long-term health risk following radiation exposure. Health Phys. 98, 109–117 (2010).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Hoffmeyer, M. R., Gillis, K., Park, J. G., Murugan, V. & LaBaer, J. Making the case for absorbed radiation response biodosimetry—Utility of a high-throughput biodosimetry system. Radiat. Res. 196, 535–546 (2021).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Hall, E. J. & Giaccia, A. J. Radiobiology for the Radiologist 8th edn. (Wolters Kluwer, 2019).
Google ScholarÂ
Garty, G., Karam, A. & Brenner, D. J. Infrastructure to support ultra-high-throughput biodosimetry screening after a radiological event. Int. J. Radiat. Biol. 87, 754–765 (2011).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Grace, M. B. et al. Rapid radiation dose assessment for radiological public health emergencies: roles of NIAID and BARDA. Health Phys. 98, 172–178 (2010).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Morana, S. J. et al. The involvement of protein phosphatases in the activation of ICE/CED-3 protease, intracellular acidification, DNA digestion, and apoptosis. J. Biol. Chem. 271, 18263–18271 (1996).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Royba, E. et al. Validation of a high-throughput dicentric chromosome assay using complex radiation exposures. Radiat. Res. 199(1), 1–16 (2022).ArticleÂ
ADSÂ
Google ScholarÂ
M’Kacher, R. et al. High resolution and automatable cytogenetic biodosimetry using in situ telomere and centromere hybridization for the accurate detection of DNA damage: An overview. Int. J. Mol. Sci. 24, 5699 (2023).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Terzoudi, G. I. et al. Dose assessment intercomparisons within the RENEB network using G0-lymphocyte prematurely condensed chromosomes (PCC assay). Int. J. Radiat. Biol. 93, 48–57 (2017).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Güttinger, S., Laurell, E. & Kutay, U. Orchestrating nuclear envelope disassembly and reassembly during mitosis. Nat. Rev. Mol. Cell Biol. 10, 178–191 (2009).ArticleÂ
PubMedÂ
Google ScholarÂ
Ghosh, S., Paweletz, N. & Schroeter, D. Failure of kinetochore development and mitotic spindle formation in okadaic acid-induced premature mitosis in HeLa cells. Exp. Cell Res. 201, 535–540 (1992).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Pines, J. & Hunter, T. Isolation of a human cyclin cDNA: Evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2. Cell 58, 833–846 (1989).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Brown, N. R. et al. CDK1 structures reveal conserved and unique features of the essential cell cycle CDK. Nat. Commun. 6, 6769 (2015).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Cremer, T. & Cremer, C. Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat. Rev. Genet. 2, 292–301 (2001).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Cremer, T. & Cremer, M. Chromosome territories. Cold Spring Harb. Perspect. Biol. 2, a003889–a003889 (2010).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Pennarun, G., Picotto, J. & Bertrand, P. Close ties between the nuclear envelope and mammalian telomeres: Give me shelter. Genes (Basel). 14, 775 (2023).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Crabbe, L., Cesare, A. J., Kasuboski, J. M., Fitzpatrick, J. A. J. & Karlseder, J. Human telomeres are tethered to the nuclear envelope during postmitotic nuclear assembly. Cell Rep. 2, 1521–1529 (2012).ArticleÂ
CASÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Hatzi, V. I. et al. The use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material. Sci. World J. 6, 1174–1190 (2006).ArticleÂ
Google ScholarÂ
Heng, H. H. Q. et al. Karyotype heterogeneity and unclassified chromosomal abnormalities. Cytogenet. Genome Res. 139, 144–157 (2013).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Kimura, K., Rybenkov, V. V., Crisona, N. J., Hirano, T. & Cozzarelli, N. R. 13S condensin actively reconfigures DNA by introducing global positive writhe: Implications for chromosome condensation. Cell 98, 239–248 (1999).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Kimura, K. & Hirano, T. ATP-dependent positive supercoiling of DNA by 13S condensin: A biochemical implication for chromosome condensation. Cell 90, 625–634 (1997).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Kanda, R., Eguchi-Kasai, K. & Hayata, I. Phosphatase inhibitors and premature chromosome condensation in human peripheral lymphocytes at different cell-cycle phases. Somat. Cell Mol. Genet. 25, 1–8 (1999).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Lorat, Y. et al. Nanoscale analysis of clustered DNA damage after high-LET irradiation by quantitative electron microscopy—The heavy burden to repair. DNA Repair (Amst). 28, 93–106 (2015).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Genzen, J. R., Mohlman, J. S., Lynch, J. L., Squires, M. W. & Weiss, R. L. Laboratory-developed tests: A legislative and regulatory review. Clin. Chem. 63, 1575–1584 (2017).ArticleÂ
CASÂ
PubMedÂ
Google ScholarÂ
Liu, C. et al. A comparison of chromosome repair kinetics in G0 and G1 reveals that enhanced repair fidelity under noncycling conditions accounts for increased potentially lethal damage repair. Radiat. Res. 174, 566–573 (2010).ArticleÂ
ADSÂ
CASÂ
PubMedÂ
Google ScholarÂ
Hu, Q. et al. Resting T cells are hypersensitive to DNA damage due to defective DNA repair pathway. Cell Death Dis. 9, 662 (2018).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Pathak, R., Ramakumar, A., Subramanian, U. & Prasanna, P. G. Differential radio-sensitivities of human chromosomes 1 and 2 in one donor in interphase- and metaphase-spreads after 60Co γ-irradiation. BMC Med. Phys. 9, 6 (2009).ArticleÂ
PubMedÂ
PubMed CentralÂ
Google ScholarÂ
Gao, L., Lu, X., Liu, M.-M., Li, S. & Liu, Q.-J. Transformed cell ratio (TCR): A novel parameter for radiation dose estimation in rapid premature chromosome condensation (PCC) assay induced by 0–40 Gy Co-60 Gamma Rays. Health Phys. 123, 492–496 (2022).ArticleÂ
CASÂ
Google ScholarÂ