This session was presented at the 2024 World Congress on In Vitro Biology Meeting held in St. Louis, Missouri from June 8 – 12, 2024 and was recorded as part of the In-Person and On-Demand Limited Access Program. Single cell RNA sequencing (SC-RNA-seq) is the state-of-the-art technology for transcriptome analysis that allows the dissection of gene expression at single-cell resolution, which greatly revolutionizes transcriptomic studies that previously analyzed RNA expression from mixtures of bulk tissues. SC-RNA‐seq can unravel the heterogeneity and complexity of RNA transcripts within individual cells, as well as revealing the composition of different cell types and functions within highly organized tissues/organs/organisms. The typical workflow consists of experimental design, sample and library preparations, sequencing, and data analysis. This workshop aims to provide students with the knowledge of basic skills and techniques used in the typical workflow to make the best decisions while implementing their SC-RNA-seq experiments. Students will be provided hands-on experience with real data sets to analyze, graph, and learn how to interpret the data. Learn more about the SIVB Annual Meetings at https://sivb.org/meetings.